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Protocol for Extraction and Electron Microscopy Visualization of Lipids in Viburnum tinus Fruit Using Cryo-Ultramicrotomy

Abstract : We recently reported that Viburnum tinus fruit generates its metallic blue color using globular lipid inclusions embedded in its epicarpal cell walls. This protocol describes steps to visualize the lipidic nature of the nanostructure using cryo-ultramicrotomy, chloroform extraction, and transmission electron microscopy (TEM) imaging. This method is useful to localize and characterize novel lipidic nanostructures embedded in both plant and animal tissues at the TEM resolution.
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https://hal.archives-ouvertes.fr/hal-03431815
Contributor : Yu Ogawa Connect in order to contact the contributor
Submitted on : Tuesday, November 16, 2021 - 10:38:02 PM
Last modification on : Thursday, December 2, 2021 - 2:14:02 PM
Long-term archiving on: : Thursday, February 17, 2022 - 9:29:32 PM

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Miranda Sinnott-Armstrong, Silvia Vignolini, Yu Ogawa. Protocol for Extraction and Electron Microscopy Visualization of Lipids in Viburnum tinus Fruit Using Cryo-Ultramicrotomy. STAR Protocols, Elsevier, 2020, 1, ⟨10.1016/j.xpro.2020.100201⟩. ⟨hal-03431815⟩

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